MitoXpress® Xtra Oxygen Consumption Assay is our leading assay kit designed as all our kits to be highly flexible in a 96- or 384-well format for a fluorescence plate reader-based approach, utilized for the direct, real-time analysis of cellular respiration and mitochondrial function. The easy-to-use MitoXpress® Xtra assay allows measurement of extracellular oxygen consumption rates (OCR) with whole cell populations (both adherent and suspension cells), isolated mitochondria, permeabilised cells and a wide range of 3D cultures including: tissues, small organisms, spheroids, scaffolds and matrixes. The assay is also suitable for measurement of isolated enzymes, bacteria, yeasts and moulds.
In this assay, MitoXpress® Xtra is quenched by oxygen through molecular collision, and thus the amount of fluorescence signal is inversely proportional to the amount of extracellular oxygen in the sample. Rates of oxygen consumption are calculated from the changes in fluorescence signal over time. The reaction is non-destructive and fully reversible (neither MitoXpress® Xtra nor oxygen are consumed), facilitating measurement of time courses and drug treatments.
Luxcel’s flexible plate reader format, allows multiparametric combination with Luxcel’s other products, as well as combining with commonly available reagents to measure glycolysis, LDH, JC-1, MMP (Ѱ), ROS, and cellular ATP. For example, MitoXpress® Xtra in combination with Luxcel’s first companion kit, the MitoXpress® FAO Assay facilitates the measurement of endogenous and exogenous fatty acids.