Oxygen: too much of a good thing? | Luxcel Biosciences
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MitoXpress® Intra – Intracellular Oxygen Assay

The only microplate-based assay kit in the world to measure cellular oxygenation.

Oxygen Concentration in Cell Cultures

Oxygen is essential for life, and while we live in an environment with O2 at approximately 21%, cells in vivo experience far lower oxygen concentrations. Microelectrode measurements suggest that tissues experience oxygen concentrations between 0.2 and 10 % dependant on tissue, despite this, the vast majority of biological research is conducted at 21% O2, a hyperoxic state for almost all cell types. This can have profound implications on the metabolism and signalling of cells in vitro and may have even led to the selection of cell lines that, while capable of surviving in a hyperoxic environment, do not faithfully reflect the in vivo condition thereby widening the gap between lab bench and clinic. Improving the physiological relevance on in vitro cell models, therefore, requires careful consideration of not just the oxygen concentration cells are cultured and tested at, but of the actual oxygen concentration experienced by those cells, a parameter with researchers are invariability blind to.

The MitoXpress® Intra Intracellular Oxygen Assay solves this problem and is the only available assay enabling cellular oxygenation measurements on a conventional fluorescence plate reader. The assay uses cell-penetrating oxygen-sensitive nanosensors which, after loading, allow real-time oxygenation measurements in both 2D and 3D cultures, thereby providing important new insights into the interrelationships between oxygen availability, metabolic adaptation and cell signalling. Importantly these nanosensors can also be combined with other fluorescent probes, facilitating multiparametric measurements of key metabolic parameters. This includes a combination with Luxcel Biosciences’ pH-XtraTM Glycolysis Assay, which reveals the impact of reduced oxygen on glycolytic flux, an interaction of profound importance in the study of tumour metabolism.

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