MitoXpress® Stress Test
Luxcel Biosciences’ MitoXpress® Stress Test is designed for use as a companion kit to the MitoXpress® Xtra Oxygen Consumption Assay and contains all the additional components necessary for the evaluation of the main characteristics of mitochondrial function. It includes Oligomycin as an Inhibitor of ATP-linked Oxygen Consumption, FCCP as an uncoupler to increase Mitochondrial Oxygen Consumption and Antimycin A to discriminate Respiratory from Non-Respiratory Oxygen Consumption.
Using this kit in conjunction with the MitoXpress® Xtra Oxygen Consumption Assay you can easily measure:
Real-time oxygen consumption measurement
Full mitochondrial characterisation – at a glance
Figure 2: Full characterisation of mitochondrial function using the MitoXpress® Stress Test in combination with the MitoXpress® Xtra Oxygen Consumption Assay. a) The slopes (m) from the linear portion of the Signal Profile from each Stress Test condition reflect Basal Respiration, Non ATP-Coupled Oxygen Consumption, Maximal Respiration and Non Respiratory Oxygen Consumption. ATP-Coupled Oxygen Consumption and Spare Respiratory Capacity are calculated from these values as described in detail in the in the MitoXpress® Stress Test Manual. b) The proportion of each of these discrete metabolic processes to the Maximal Respiration can be conveniently visualised producing a detailed picture of aerobic metabolism.